Washington University in St. Louis
Campus Box 1137
One Brookings Drive
St. Louis, MO 63130-4899
- Protein Assembly
- Gene Regulation
The Kranz group studies two different processes that occur in bacteria and plants, using various model organisms that are best suited for each process:
i. Cytochrome c biogenesis pathways (see the figure). Using biochemical, genetic, and immunological approaches, the group studies a photosynthetic bacterium, Rhodobacter capsulatus, for System I and Bordetella pertussis as a model for System II. Recombinant E.coli with each system are also used.
ii. The control of gene expression in response to nitrogen in the environment, using R. capsulatus and Arabidopsis.
Photo caption: Three unique systems have evolved to assemble the c-type cytochromosomes. The assembly proteins for each system are shown. (c-type cytochromes have heme that is covalently linked to two cysteines.)
Richard-Fogal, C.L., E. R. Frawley, E. R. Bonner, H. Zhu, B. San Francisco*, and R. G. Kranz. 2009. A conserved haem redox and trafficking pathway for cofactor attachment. EMBO J. 28 (16): 2349-2359.
Kranz, R.G., C. Richard-Fogal, J. S. Taylor, E. R. Frawley. 2009. Cytochrome c biogenesis: mechanisms for covalent modifications and trafficking of heme, and for heme-iron redox control. MMBR. 73(3); 510-528. (A REVIEW)
Frawley ER, Kranz RG. 2009. CcsBA is a cytochrome c synthetase that also functions in heme transport. Proc Natl Acad Sci U S A 106(25):10201-6.
Zhu, H, Fitzsimmons, K, Khandelwal, A, Kranz, RG. 2009. CPC, a single-repeat R3 MYB, is a negative regulator of anthocyanin in Arabidopsis, Molecular Plant. June 2, 2009, doi:10.1093/mp/ssp030.
Turkarslan, S, DW Lee, O Onder, RG Kranz, F Daldal . 2008. The cytochrome c maturation components CcmF, CcmH and CcmI form a membrane-integral multisubunit complex - J Biol Chem. 283 (44): 29715-29722.
Richard-Fogal CL, Frawley ER, Kranz RG. 2008. Topology and function of CcmD in cytochrome c maturation. J Bacteriol. 2008 May;190(10):3489-93.
Richard-Fogal C.L., Frawley E.R., Feissner R.E. , and Kranz R.G. 2007. Heme concentration dependence and metalloporphyrin inhibition of the system I and II cytochrome c assembly pathways. J. Bacteriol. 189(2):455-63.