Washington University in St. Louis
Campus Box 1137
One Brookings Drive
St. Louis, MO 63130-4899
DBBS graduate programs Developmental, Regenerative and Stem Cell Biology Program
Molecular Cell Biology Program
The bone cell microenvironment consists of bone forming osteoblasts, bone resorbing osteoclasts and thei precursors, as well as immune and vascular cells. Complex endocrine and local signals are integrated to mediate bone cell development and function. This group's research focuses on understanding select aspects of the cell and molecular interactions that are required for normal bone development as well as those that may contribute to age-associated and inflammatory-mediated pathological bone loss.
Photo caption: Panel on top shows scanning electron micrograph of isolated osteoclast resorbing bone. Panel on bottom is a cartoon depicting cytokine and cell interactions that may contribute to recruitment, differentiation, and activation of bone resorbing osteoclasts (depicted in red).
Sunyer, T., Lewis, J., Collin-Osdoby, P., Osdoby, P. (1999) Estrogen inhibits interleukin-1 response of human marrow-derived osteoclast-like cells by modulating isoforms of IL-1 receptor expression. J. Clin. Invest. 103: 1409-1418.
Collin-Osdoby, P., Rothe, L., Becker, S., Anderson, F., Osdoby, P. (2000) Decreased nitric oxide levels promote osteoclast formation and bone resorption in chick bone marrow and isolated osteoclast cultures in vitro as well as in vivo on the chick chorioallantoic membrane in conjunction with neoangiogenesis. J. Bone Min. Res. 15: 474-488.
Wright, L., Beck, S., Schwent, B., Anderson, F., Moura, P., Nelson, M., Maloney, W., Collin-Osdoby, P., Osdoby, P. (2000) The chemokine receptor CXCR4 and its ligand, SDF-1, are involved in human osteoclast SDF-1, are involved in human osteoclast. Submitted to J. Clin. Invest.
Collin-Osdoby, P., Rothe, L., Anderson, F., Nelson, M., Maloney, W., Osdoby, P. (2001) Receptor activator of NF-kappa B and osteoprotegerin expression by human microvascular endothelial cells, regulation by inflammatory cytokines, and role in human osteoclastogenesis. J. Biol. Chem. 276(23): 20659-20672.
Gorski, J., Liu, F., Artigues, A., Castagna, L., and Osdoby, P. (2002) New alternatively spliced form of galectin-3, a new member of the beta-galactosidase-binding animal lectin family, contains a predicted transmembrane-spanning domain and a leucine zipper motif. J Biol. Chem. 277: 18840-18848.
Collin-Osdoby, P., Rothe, L., Bekker, S., Anderson, F., Huang, Y., and Osdoby, P. (2002) Basic fibroblast growth factor stimulates osteoclast recruitment, development, and bone pit resorption in association with angiogenesis in vivo on the chick chorioallantoic membrane and activates isolated avian osteoclast resorption in vitro. J. Bone Miner. Res. 17:1859-1871.
Yu X, Huang Y, Collin-Osdoby P, Osdoby P, (2003) Stromal cell-derived factor-1(SDF-1) recruit osteoclast precursors by inducing chemotaxis, matrix metalloproteinase-9 (MMP-9) activity, and collagen transmigration. Journal of Bone and Mineral Research. 18 1404-1418.
Collin-Osdoby P, Yu X, Zheng H, Osdoby P. (2003) RANKL-mediated osteoclast formation from murine RAW 264.7 cells. In: Methods in Molecular Medicine. Bone Research Protocols. S Ralston, M Helfrich, eds. Human Press, Totowa, NJ. pgs 153-166.
Yu X, Huang Y, Collin-Osdoby P, Osdoby P. (2004) CCR1 chemokines promote the chemotactic recruitment, RANKL development, and motility of osteoclasts and are induced by inflammatory cytokines in osteoblasts. Bone Miner Res 19:2065-2077.
Wright L, Maloney W, Yu X, Kindle L, Collin-Osdoby P, Osdoby P. (2005) Stromal cell-derived factor-1 binding to its chemokine receptor CXCR4 on precursor cells promotes the chemotactic recruitment, development and survival of human osteoclasts. Bone. 2005;36(5):840-53. 2005
Saltman L, Javed A, Ribadeneyra J, Hussain S, Young D, Osdoby P, Amcheslavsky A, van Wijnen A, Stein J, Stein G, Lian J, Bar-Shavit Z (2005) Organization of transcriptional machinery and regulatory factors in osteoclasts nuclei: Compartmentalization of Runx1. J Cell Physiol. 2005:871-80
Kindle L, Rothe L, Kriss M, Osdoby P, Collin-Osdoby P. (2006) Human microvascular endothelial cell activation by IL-1 and TNF-a stimulates the adhesion and transendothelial migration of circulating human CD14+ monocytes that develop with RANKL into functional osteoclasts. J Bone Miner Res 21: 193-206.
Zheng, H. Yu, H., Collin-Osdoby, P. Osdoby, P. (2006) RANKL Stimulates iNOS and NO production in Developing Osteoclasts via NF-kB: An Autocrine Negative Feedback Mechanism to Regulate Osteoclastogenesis and Bone Resorption. J Biol Chem. ;281(23):15809-20.