Nusinow Lab Summary:
Circadian regulation of physiology and development in plants.
How do plants know what time it is and what season they are in without a watch or calendar?
Time measurement is critical in biology. Each day the Earth’s rotation causes oscillations in light and temperature with a period of approximately 24 hours. Selective pressure has led to the repeated evolution of an entrainable, endogenous timekeeper that permits for the anticipation and measurement of these daily cycles. The circadian clock allows for the resonance of internal and environmental oscillations, providing an adaptive advantage through the coordination of physiology and development with daily and seasonal change. While we monitor our watches to know when to eat, meet, and sleep, plants use their internal clock to anticipate sunrise to prepare to harvest photons, measure day-length for tracking seasonal change and to meter out resources to ensure that energy reserves last throughout the evening.
The Nusinow laboratory is focused on understanding how the circadian clock is integrated with environmental signals to control growth, physiology and development. We use a combination of molecular, biochemical, genetic, genomic, and proteomic tools to uncover the molecular connections between signaling networks, the circadian oscillator, and specific outputs (e.g. growth, photosynthesis, photoperiodism, starch accumulation, and defense). Through combining these methods with a comparative proteomics approach, we aim to leverage the knowledge gained from the model plant Arabidopsis to other plant species. Our increased understanding of the mechanisms underlying growth and development will help improve their use as feed, food, and fuel stocks.
Chow BY, Helfer A, Nusinow DA, Kay SA (2012) ELF3 recruitment to the PRR9 promoter requires other Evening Complex members in the Arabidopsis circadian clock. Plant Signaling and Behavior, Published online February 1, 2012
Nusinow DA, Helfer A, Hamilton EE, King JJ, Imaizumi T, Schultz TF, Farre EM, Kay SA (2011) The ELF4-ELF3-LUX Complex Links the Circadian Clock to Diurnal Control of Hypocotyl Growth. Nature, Jul 21;475(7356):398-402 Published online July 13, 2011.
Helfer A, Nusinow DA, Chow BY, Gehrke AR, Bulyk ML, Kay SA (2011) LUX ARRHYTHMO Encodes a Nighttime Repressor of Circadian Gene Expression in the Arabidopsis Core Clock. Current Biology, Jan 25;21(2):126-33. Published online January 13, 2011.
Zhang E, Liu Y, Dentin R, Pongsawakul PY, Liu AC, Hirota H, Nusinow DA, Sun X, Landais S, Kodama Y, Brenner D, Montminy M, Kay SA (2010) CRYPTOCHROME Mediates Circadian Regulation of cAMP Signaling and Hepatic Gluconeogenesis. Nature Medicine, Oct;16(10):1152-6. Published online September 19, 2010.
Sawa M, Nusinow DA, Kay SA, and Imaizumi T (2007) FKF1 and GIGANTEA complex formation is critical for day-length measurement in Arabidopsis. Science, 318(5848): 261-5. Published online September 13, 2007.
Nusinow DA, Hernández-Muñoz I, Fazzio T, Shah GM, Kraus WL, and Panning B (2007) Poly (ADP-ribose) polymerase 1 is inhibited by a histone H2A variant, MACROH2A, and contributes to silencing of the inactive X chromosome. Journal of Biological Chemistry, 282(17): 12851-9. Published online February 23, 2007.
Chu F, Nusinow DA, Chalkley RJ, Plath K, Panning B, Burlingame AL (2006) Mapping post-translational modifications of the histone variant macroH2A1 using tandem mass spectrometry. Molecular and Cellular Proteomics, 5(1): 194-92. Published online October 5, 2005
Hernandez-Munoz I, Lund AH, van der Stoop P, Boutsma E, Muijrers I, Verhoeven E, Nusinow DA, Panning B, Marahrens Y, van Lohuizen M (2005) Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligase. Proceedings of the National Academy of Sciences, 102(21): 7635-40. Published online May 16, 2005