Washington University in St. Louis
Campus Box 1137
One Brookings Drive
St. Louis, MO 63130-4899
DBBS graduate programs Plant and Microbial Biosciences Program
Molecular Genetics and Genomics Program
Animals require cell movement to determine the final form of organs. Because there is no morphogenic cell movement in plants, and because the cell wall is usually formed immediately after cell division, plant morphogenesis depends upon careful control of both cell division and cell expansion. The auxin indole-3-acetic acid (IAA) is a critical plant hormone, controlling both cell division and cell expansion and thereby orchestrating many developmental events and environmental responses. Normal plant morphogenesis and environmental responses require modulation of auxin levels and responsiveness by interaction with other hormones, controlling biosynthesis, regulating transport, and managing storage forms. One auxin storage form of interest to the Strader lab is the side chain-lengthened compound indole-3-butyric acid (IBA), which is shortened into IAA by peroxisomal β-oxidation.
Current research in the Strader lab focuses on several projects using the model plant Arabidopsis thaliana:
• The role of the MAP kinase phosphatase IBR5 on mediating responses to the plant hormones auxin, abscisic acid, and ethylene
• The role of IBA-derived auxin in plant development
• The role of IBA transport in plant development
Photo caption: PDR8/PEN3/ABCG36 is an ABC transporter that is required for efflux of the auxin precursor IBA out of the root tip. ABCG36-GFP is localized within epidermal (ep) and lateral root cap (LRC) cells in the root to define the root interface with the rhizosphere (Strader and Bartel, 2009). Confocal images of a root tip from 6-day-old ABCG36:ABCG36-GFP. (A) The right panel shows ABCG36-GFP; the left panel shows propidium iodide conterstaining. The stele, epidermal (ep), cortex (co), endodermal (en), quiescent center (QC), lateral root cap (LRC), and columella cells are labeled on the propidium iodide image. (B) Digital magnification of lateral root cap and epidermal cell ABCG36-GFP. Scale bar is 20 µm.
Strader, LC and Y Zhao (2016) Auxin perception and downstream events. Current Opinion in Plant Biology 33:8-14.
Powers, SK, and LC Strader (2016) Up in the air: Untethered factors of auxin response. F1000Research 5(F1000 Faculty Rev):133.
Enders, TA, S Oh, ZB Yang, B Montgomery, and LC Strader (2015) Genome sequencing of Arabidopsis abp1-5reveals second-site mutations that may affect phenotypes. The Plant Cell 27:1820-1826.
Korasick, DA, JM Jez, and LC Strader (2015) Refining the nuclear auxin response pathway through structural biology. Current Opinion in Plant Biology 27:22-28.
Korasick, DA, S Chatterjee, M Tonelli, H Dashti, SG Lee, CS Westfall, DB Fulton, AH Andreotti, GK Amarasinghe, LC Strader, and JM Jez (2015) Defining a two-pronged structural model for PB1 domain interaction in plant auxin responses. Journal of Biological Chemistry 290:12868-12878.
Michniewicz, M, EM Frick, and LC Strader (2015) A Gateway-compatible tissue-specific set of plant expression vectors. BMC- Research Reports 8:63.
Enders, TA and LC Strader (2015) Auxin: Past, present, and future. American Journal of Botany 102:180-196.
Thole, JM and LC Strader (2015) Next-generation sequencing as a tool to quickly identify causative EMS-generated mutations. Plant Signaling and Behavior 10:5, e10000167.
Korasick, DA, CS Westfall, SG Lee, R Dumas, M Nanao, G Hagen, TJ Guilfoyle, JM Jez, and LC Strader (2014) Molecular basis for AUXIN RESPONSE FACTOR interaction and the control of auxin response repression. Proceedings of the National Academy of Sciences USA 111:5427-5432.
Thole, JM, ER Beisner*, J Liu*, SV Venkova*, and LC Strader (2014) Abscisic acid regulates root elongation through the activities of auxin and ethylene. G3: Genes | Genomes | Genetics 4:1259-1274. (*undergraduate authors)
Michniewicz, M, SK Powers*, and LC Strader (2014) PDR Proteins and IBA Transport. In Plant ABC Transporters. M. Geisler, ed., 313-331. (*undergraduate author)
Korasick, DA, TA Enders, and LC Strader (2013) Auxin biosynthesis and storage forms. Journal of Experimental Botany 64:2541-2555.
Strader, LC and JL Nemhauser (2013) Auxin 2012: a rich mea ho'oulu. Development 140:1153-1157.
De Rybel, B, D Audenaert, W Xuan, P Overvoorde, LC Strader , S Kepinksi, R Hoye, R Brisbois, B Parizot, S Vanneste, X Liu, A Gilday, I Graham, L Nguyen, L Jansen, MF Njo, D Inzé, B Bartel and T Beeckman (2012) A role for the root cap in root branching revealed by the non-auxin probe naxillin. Nature Chemical Biology, 8:798-805.
Rinaldi, MA, J Liu*, TA Enders, B Bartel, and LC Strader (2012) A gain-of-function mutation in IAA16 confers reduced responses to auxin and abscisic acid and impedes plant growth and fertility. Plant Molecular Biology 79:359-373. (*undergraduate author)
Strader, LC, DL Wheeler*, SE Christensen, JC Berens*, JD Cohen, RA Rampey, and B Bartel (2011) Multiple facets of Arabidopsis seedling development require indole-3-butyric acid-derived auxin. The Plant Cell, 23:984-999. (*undergraduate authors)
Strader, LC, GL Chen*, and B Bartel (2010) Ethylene directs auxin to control cell expansion. The Plant Journal, 64:874-884. (*undergraduate author)
Strader, LC, A Hendrickson Culler, JD Cohen, and B Bartel (2010) Conversion of endogenous indole-3-butyric acid to indole-3-acetic acid drives cell expansion in Arabidopsis seedlings. Plant Physiology 153:1577-1586.
Růžička, K, LC Strader, A Bailly, H Yang, J Blakeslee, Ł Łangowski, E Nejedlá, H Fujita, H Ito, K Syōno, J Hejátko, WM Gray, E Martinoia, M Geisler, B Bartel, A Murphy, and J Friml (2010) Arabidopsis PIS1 encodes the ABCG37 transporter of auxinic compounds including the auxin precursor indole-3-butyric acid. Proceedings of the National Academy of Sciences USA 107:10749-10753.
Strader, LC, ER Beisner*, and B Bartel (2009) Silver ions increase auxin efflux independently of effects on ethylene response. The Plant Cell 21:3585-3590. (*undergraduate author)
Strader, LC, and B Bartel (2009) The Arabidopsis PLEIOTROPIC DRUG RESISTANCE8/ABCG36 ATP binding cassette transporter modulates sensitivity to the auxin precursor indole-3-butyric acid. The Plant Cell 21:1992-2007.
Strader, LC, M Monroe-Augustus, KC Rogers*, GL Lin*, and B Bartel. (2008) Arabidopsis iba response5 (ibr5) suppressors separate responses to various hormones. Genetics 180:2019-2031. (*undergraduate authors)
Strader, LC and B Bartel (2008) A new path to auxin. Nature Chemical Biology 4:337-339.